The Archives of Bone and Joint Surgery

The Archives of Bone and Joint Surgery

Matrix Metalloproteases 8 Polymorphism as Risk Factor for Rotator Cuff Tear

Document Type : RESEARCH PAPER

Authors
Nathali Nunes Cavascan 1
Jorge Henrique Assunção 2
Alexandre Leme Godoy-Santos 2
Arnaldo Amado Ferreira Neto 2
Maria Cristina Leme Godoy Santos 1
1 Department of Cell Biology, University Federal of Paraná, Curitiba, PR, Brazil
2 Department of Orthopedics and Traumatology, University of São Paulo, São Paulo, SP, Brazil
10.22038/abjs.2023.55108.2743
Abstract
Objectives: Rotator Cuff Tear (RCT) is a multifactorial disease, but an important one is the increased 
collagen degradation that would lead to a higher chance of tear. MMP-8 is a protein that degrades type 
I collagen, and it is known that MMP-8 has a polymorphism in which a T allele in the gene promoter 
region increases its transcription activity. This study aims to investigate the association between MMP -
8 polymorphism g.-799 C>T (rs11225394) and RCT.
Methods: To do that, we collected DNA samples from buccal epithelial cells of 128 patients (separated into RCT 
group and control group in a proportion 1:1) and genotyped the DNA using PCR. The statistical analyses were done 
using the ARLEQUIN Version 2.0, and the data normality was tested with the Shapiro-Wilk test.
Results: The results showed a significantly higher frequency of T/T genotype in the test group (29% in the control 
group and 39% in the test group, p=0.0417), and that would represent a risk factor for increased collagen 
degradation.
Conclusion: The MMP-8 g.-799 C>T (rs11225394) SNP was associated with RCT. With the description of a new 
risk factor, future research can be done to analyze how to prevent RCT or develop new treatment strategies since 
the disease's failure index is currently high.
 Level of evidence: II
Keywords
Genetic polymorphism
Metalloproteases
Risk factor
Rotator cuff tear
Subjects
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The Archives of Bone and Joint Surgery
Volume 11, Issue 9
September 2023
Pages 565-569

  • Receive Date 08 April 2021
  • Revise Date 30 May 2023
  • Accept Date 15 March 2023