Histomorphometric Analysis of Newly-formed Bone Using Octacalcium Phosphate and Bone Matrix Gelatin in Rat Tibial Defects

Document Type: RESEARCH PAPER

Authors

1 Cellular and Molecular Research Center & Department of Anatomical Sciences, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

2 Department of Community Medicine, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

3 Department of Anatomical Sciences, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran

4 Department of Pathology, University of Washington, Seattle, Washington, USA

Abstract

Background: Repair of bone defects is challenging for reconstructive and orthopedic surgeons. In this study, we aimed
to histomorphometrically assess new bone formation in tibial bone defects filled with octacalcium phosphate (OCP),
bone matrix gelatin (BMG), and a combination of both.
Methods: A total of 96 male Sprague Dawley rats aged 6-8 weeks weighing 120-150 g were randomly allocated
into three experimental (OCP, BMG, and OCP/BMG) and one control group (n=24 in each group). The defects in
experimental groups were filled with OCP (6 mg), BMG (6 mg), or a combination of OCP and BMG (6 mg, 2:1 ratio).
No material was used to fill the defects in the control group and the defect was only covered with Surgicel. Samples
were taken on days 7, 14, 21, and 56 after the surgery. The sections were stained with hematoxylin-eosin (H&E) and
assessed using light microscopy.
Results: In our experimental groups, bone formation was started from the margins of the defect towards the center
with an increasing rate during the study period. Moreover, the formed bone was more mature. Bone formation in our
control group was only limited to the margins of the defect. The newly formed bone mass was significantly higher in
the experimental groups (P=0.001).
Conclusion: OCP, BMG, and OCP/BMG compound enhanced osteoinduction in long bones.
Level of evidence: III

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